Cyclooxygenase-2 expression is suppressed by eicosapentaenoic acid and is a prognostic factor for esophageal squamous cell carcinoma patients after curative surgery

Main Article Content

Koji Mizoguchi Hideyuki Ishiguro Ryo Ogawa Tatsuya Tanaka Hiroyuki Sagawa Tomotaka Okubo Yosuke Samoto

Abstract

Background/Purpose

We showed that eicosapentaenoic acid (EPA) stimulated apoptosis via caspases in ESCC cell lines, indicating an anti-cancer effect. In the present study, we aimed to clarify whether the anticancer and anti-inflammatory actions of EPA are related to COX-2.

Materials and method

We performed immunohistochemical analysis in 92 ESCC specimens to investigate the relationship between COX-2 expression and prognosis, and Western blotting in ESCC cell lines to determine whether EPA suppresses COX-2.

Results

Immunohistochemistry showed that COX-2 expression levels were enhanced in the ESCC specimens, with a significant prognostic impact on the survival of surgically treated cases (P=0.0017, log-rank test). Multivariate analysis showed that the prognostic impact of COX-2 is independent of disease stage (P=0.02). Western blotting revealed that the KYSE180 and TE11 ESCC cell lines express COX-2, which was greatly suppressed by EPA stimulation.

Conclusions

The expression of COX-2 in patients with ESCC is associated with significantly poor survival rates. EPA may have an anti-cancer effect via suppression of COX-2.

Article Details

How to Cite
MIZOGUCHI, Koji et al. Cyclooxygenase-2 expression is suppressed by eicosapentaenoic acid and is a prognostic factor for esophageal squamous cell carcinoma patients after curative surgery. Medical Research Archives, [S.l.], v. 5, n. 3, mar. 2017. ISSN 2375-1924. Available at: <https://journals.ke-i.org/mra/article/view/1024>. Date accessed: 04 aug. 2020.
Keywords
cyclooxygenase-2, eicosapentaenoic acid, esophageal squamous cell carcinoma, immunohistochemistry,  prognosis
Section
Research Articles

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