Cyclooxygenase-2 expression is suppressed by eicosapentaenoic acid and is a prognostic factor for esophageal squamous cell carcinoma patients after curative surgery

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Koji Mizoguchi Hideyuki Ishiguro Ryo Ogawa Tatsuya Tanaka Hiroyuki Sagawa Tomotaka Okubo Yosuke Samoto



We showed that eicosapentaenoic acid (EPA) stimulated apoptosis via caspases in ESCC cell lines, indicating an anti-cancer effect. In the present study, we aimed to clarify whether the anticancer and anti-inflammatory actions of EPA are related to COX-2.

Materials and method

We performed immunohistochemical analysis in 92 ESCC specimens to investigate the relationship between COX-2 expression and prognosis, and Western blotting in ESCC cell lines to determine whether EPA suppresses COX-2.


Immunohistochemistry showed that COX-2 expression levels were enhanced in the ESCC specimens, with a significant prognostic impact on the survival of surgically treated cases (P=0.0017, log-rank test). Multivariate analysis showed that the prognostic impact of COX-2 is independent of disease stage (P=0.02). Western blotting revealed that the KYSE180 and TE11 ESCC cell lines express COX-2, which was greatly suppressed by EPA stimulation.


The expression of COX-2 in patients with ESCC is associated with significantly poor survival rates. EPA may have an anti-cancer effect via suppression of COX-2.

Article Details

How to Cite
MIZOGUCHI, Koji et al. Cyclooxygenase-2 expression is suppressed by eicosapentaenoic acid and is a prognostic factor for esophageal squamous cell carcinoma patients after curative surgery. Medical Research Archives, [S.l.], v. 5, n. 3, mar. 2017. ISSN 2375-1924. Available at: <>. Date accessed: 04 aug. 2020.
cyclooxygenase-2, eicosapentaenoic acid, esophageal squamous cell carcinoma, immunohistochemistry,  prognosis
Research Articles


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