TY - JOUR AU - Carvalho, Rodrigo Dias de Oliveira AU - Morais, Kátia AU - Pereira, Vanessa Bastos AU - Gomes-Santos, Ana Cristina AU - Luerce, Tessalia Diniz AU - de Azevedo, Marcela Santiago Pacheco AU - Rocha, Clarissa Santos AU - de Sousa, Cassiana Severiano AU - Prósperi, Camila AU - Cara, Denise Carmona AU - Faria, Ana Maria Caetano Faria Caetano AU - Bermudez-Humaran, Luis AU - Blottiere, Hervé AU - Langella, Philippe AU - de Moreno, Alejandra AU - Figueiredo, Henrique Cesar Pereira AU - LeBlanc, Jean Guy AU - Miyoshi, Anderson AU - Azevedo, Vasco PY - 2016/11/17 TI - Oral administration of Lactococcus lactis expressing recombinant 15-lipoxygenase-1 (15 LOX-1) modulates chemically induced colitis in mice JF - Medical Research Archives; Vol 4 No 7 (2016): Vol.4 Issue 7, November 2016 KW - colitis, inflammation, lactic acid bacteria, Lactococcus lactis, lipoxygenase, imunomodulation N2 - Abstract   Background : Inflammatory bowel disease (IBD), such as Crohn’s disease and ulcerative colitis, are characterized by extensive inflammation due to dysregulation of the innate and adaptive immune system whose exact etiology is not yet completely understood. Currently there is no cure for IBD, thus the search for new molecules capable of controlling IBD and their delivery to the site of inflammation are the goal of many researchers. The aim of this work was to evaluate the anti-inflammatory effect of the oral administration of a Lactococcus (L.) lactis  strain producing 15-lipoxygenase-1 (15-LOX-1) using a dextran sodium sulfate (DSS)-induced IBD mouse model. Methods: The anti-inflammatory strain L. lactis lacti NCDO 2118 was modified to produce active 15-LOX-1 and tested in in a DSS induced IBD mouse model. Results: This 15-LOX-1 producing L. lactis  was effective in the prevention of the intestinal damage associated to inflammatory bowel disease in a murine model and decreased pro-inflammatory cytokines such as IFN-γ and IL-4 while increasing the anti-inflammatory cytokine IL-10. L. lactis  NCDO 2118 acts not just as a tool to delivery 15-LOX-1 in the inflamed gut mucosa but still retains its anti-inflammatory effects. Conclusions: This strain could be used in novel adjunct IBD treatment protocols. UR - https://esmed.org/MRA/mra/article/view/612