Effects of Light Emitting Diode and Low-intensity Light on the immunological process in a model of Parkinson's disease

Main Article Content

Afonso Shiguemi Salgado Larissa Gulogurski Ribeiro Thais Barbosa Oliveira Marcos Paulo Rolão Jossinelma Camargo Gomes Emerson . Carraro Mário César Perreira Patricia Tyski Suckow Ivo Ilvan Kerppers

Abstract

Parkinson's disease (PD) is characterized primarily by the loss of dopaminergic neurons in the substantia nigra and appearance of alpha-synuclein aggregates in Lewy bodies. The neuroinflammation in Parkinson's disease is associated with activation of microglia, the participation of inflammatory cytokines, and systemic activation of natural killer cells. Evidence suggests that several inflammatory cytokines are enhanced in the brain and blood of patients presenting with Parkinson's disease. In addition, others studies have suggested that Light Emitting Diode (LED) and Low-intensity Light (Laser) hold potential for improving neuronal cell function in patients with Parkinson's Disease. This study investigated the influence of LED and Laser on inflammatory processes caused by an electrolytic lesion of the substantia nigra in an experimental model of Parkinson's disease. Sixty Wistar rats were divided into three experimental groups (LED, Laser, and Control). An electrode was placed in the cortex for PD induction that was evaluated for motor conditions after 30 days in an Open Field. Cytokines levels were analyzed by flow cytometry using the BD Cytometric Bead Array Mouse Th1/Th2 Cytokine Kit. Serum cytokine concentrations (IL-4, IL-6, IL-10, TNF-α, IFN-γ) were significantly different in each group. When compared with other groups, the concentrations of interferon and IL-2 were higher in the LED  and Laser groups, respectively.  TNF-α showed lower concentrations in the LED and Laser groups when compared with the Control group. The LED and Laser actions on the central nervous system in an animal model with lesions presenting neurodegeneration and persistent inflammation, such as in Parkinson's disease, show significant effects for the treatment and prevention of neurodegeneration caused by pro-inflammatory cytokines.

Article Details

How to Cite
SALGADO, Afonso Shiguemi et al. Effects of Light Emitting Diode and Low-intensity Light on the immunological process in a model of Parkinson's disease. Medical Research Archives, [S.l.], v. 4, n. 8, dec. 2016. ISSN 2375-1924. Available at: <https://journals.ke-i.org/index.php/mra/article/view/652>. Date accessed: 25 mar. 2019.
Keywords
: Parkinson's disease, cytokines, LED, Laser
Section
Research Articles

References

1. de Mattos LH, Álvarez LE, Yamada AL et al. (2015) Effect of phototherapy with light-emitting diodes (890 nm) on tendon repair: an experimental model in sheep. Lasers Med Sci. 30:193–201.

2. Gomoll AH, Katz JN, Warner JJ et al.(2004) Rotator cuff disorders: recognition and management among patients with shoulder pain. Arthritis Rheum. 50:3751–61

3. Helrigle C, Carvalho PTC, Casalechi HL et al. (2015) Effects of low-intensity non-coherent light therapy on the inflammatory process in the calcaneal tendon of ovariectomized rats. Lasers Med Sci. 15:182-7.

4. Giugno K et al. (2004) Concentrations of interleukin-2 in the nasopharyngeal secretion of children with acute respiratory syncytial virus bronchiolitis. Jornal de pediatria. 80:315-20.
5. Novoselova EG et al. (1989) Effects of low‐power laser radiation on mice immunity Photodermatology, photoimmunology & photomedicine. 22: 33-8.
6. Mizel B. (1989) The interleukins The FASEB Journal. 3: 2379-88.
7. Han A, Yeol H, Park MJ et al. (2015) IL-4/10 prevents stress vulnerability following imipramine discontinuation. Journal of Neuroinflammation. 12:197.

8. Blume J, Douglas SD, Evans DL. (2011) Immune suppression and immune activation in depression. Brain Behav Immun. 25:221–9.

9. Dantzer R, O’Connor JC, Lawson MA et al. (2011) Inflammation-associated depression: From serotonin to kynurenine. Psychoneuroendocrinology, 36:426–36.

10. Miller AH, Maletic V, Raison CL.(2009) Inflammation and its discontents: the role of cytokines in the pathophysiology of major depression. Biol Psychiatry. 65:732–41.

11. Henningsson AJ, Tjernberg I, Malmvall BO et al. (2011) Indications of Th1 and Th17 responses in cerebrospinal fluid from patients with Lyme neuroborreliosis: a large retrospective study. Journal of Neuroinflammation. 8:36.

12. Mogi M, Harada M, Kondo T et al. (1994) Interleukin-2 but not basic fibroblast growth factor is elevated in parkinsonian brain. J Neural Transm. 103:1077–81.

13. Mogi M, Harada M, Narabayashi H et al. (1996) Interleukin (IL)-1 beta, IL-2, IL-4, IL-6 and transforming growth factor-alpha levels are elevated in ventricular cerebrospinal fluid in juvenile parkinsonism and Parkinson’s disease. Neurosci Lett. 211:13–6.

14. Bialecka, M, Klodowska-Duda, G, Kurzawski, M et al. (2008) Interleukin-10 (IL10) and tumor necrosis factor α (TNF) gene polymorphisms in Parkinson’s disease patients. Parkinsonism Relat Disord. 14: 636–640.

15. Catão MHCV. (2004) Os benefícios do laser de baixa intensidade na clínica odontológica na estomatologia. Rev Bras Patol Oral. 3:214-8.
16. Paxinos, G., Franklin, K.B.J., 2004. The Mouse Brain in Stereotaxic Coordinates. Second Editiion. Elsevier. 136 p.

17. Betancourt, E., Wachtel, J., Michaelos, M., Haggerty, M., Conforti, J., Kritzer, M.F., 2016. The impacto f biological sex and sex hormones on cognition in rat modelo f early, pre-motor Parkinson´s Disease. doi:10.1016/j.neuroscience.2016.05.041

18. Rocha Junior AM, Vieira B.J, Andrade LCF et al. (2007) Effects of low-level laser therapy on the progress of wound healing in humans: the contribution of in vitro and in vivo experimental studies. J Vasc Bras. 6:258-66.

19. Zhevago N., Samoilova, K. (2006) Pro- and Anti-inflammatory Cytokine Content in Human Peripheral Blood after Its Transcutaneous (in Vivo) and Direct (in Vitro) Irradiation with Polychromatic Visible and Infrared Light. Photomedicine and Laser Surgery. 24:129-139.

20. Kraychete D.C., Calasans M.T.A., Valente, C.M.L. (2006) Citocinas Pró-inflamatórias e Dor.Rev Bras Reumatol. 46:199-206.

21. Novoselova EG et al. (1989) Effects of low‐power laser radiation on mice immunity Photodermatology, photoimmunology & photomedicine. 22: 33-38.

22. Akira S et al. (1990) Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF) The FASEB journal. 4: 2860-2867.

23. Carlos F. Estudo da fototerapia de baixa potência no modelo de inflamação aguda induzida no joelho de ratos. 2011. 48 f. Dissertação (Mestrado em Ciências da Reabilitação) – Universidade Nove de Julho, São Paulo. 2011.

24. Moreira MSN Tese: analise da biomodulação da inflamação apos lesão criogênica no sistema nervoso central em ratos submetidos a fototerapia com laser de baixa intensidade. São Paulo, 2010.

25. Eskdale J, Gallagher G. A (1995) polymorphic dinucleotide repeat in the human IL-10 promoter. Immunogenetics. 42:444-445.

26. Rentzos M, Nikolaou C, Andreadou E et al. (2009) Circulating interleukin-10 and interleukin-12 in Parkinsons disease. Acta Neurol Scand. 119: 332–337

27. Choi L, Kim K, Ko K et al. (2012) Inflammatory cytokines are suppressed by light-emitting diode irradiation of P. gingivalis LPS-treated human gingival fibroblastos. Lasers Med Sci. 27:459–467.

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.