Reversal of neuromuscular block produced by local anaesthetics combined with pancuronium. Experimental study.

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1776 - pdf - 20.04.2018
Published May 18, 2018
DOI: https://doi.org/10.18103/mra.v6i5.1776

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Vanessa Henriques Carvalho
State University of Campinas, São Paulo, Brazil
Angélica Assunção Braga
State University of Campinas, São Paulo, Brazil
Franklin Sarmento Braga
State University of Campinas, São Paulo, Brazil
Fernando Eduardo Feres Junqueira
State University of Campinas, São Paulo, Brazil
Renata Muzzi Drummond
State University of Campinas, São Paulo, Brazil
Guilherme Litterio Benette
State University of Campinas, São Paulo, Brazil

Abstract

Abstract


Justification and objectives: There are few clinical and experimental studies on the interaction between local anaesthetics and nondepolarising neuromuscular blocking agents and their effects on the neuromuscular junction. However, potentiation of these drugs has been observed when they are used simultaneously. The aim of this study was to evaluate in vitro the efficacy of neostigmine and 4-aminopyridine in the reversal of a neuromuscular block produced by pancuronium combined with different local anaesthetics.


Method: Rats were distributed in groups (n = 5), according to drug combinations: pancuronium + levobupivacaine and pancuronium + enantiomeric mixture. The concentrations of levobupivacaine, enantiomeric mixture and pancuronium were respectively 5µg.mL-1, 5µg.mL-1 and 2µg.mL-1. Neostigmine and 4-aminopyridine were used in concentrations of 2µg.mL-1 and 20µg.mL-1, respectively. The following parameters were evaluated: 1) degree of neuromuscular block obtained by a combination of pancuronium + levobupivacaine and pancuronium + enantiomeric mixture; 2) efficacy of neostigmine and 4-aminopyridine in the reversal of neuromuscular block produced by drug combinations.


Results and Conclusion: Neostigmine and 4-aminopyridine were effective at reversing neuromuscular block obtained by different drug combinations, and the block was partially and totally reversed with neostigmine and 4-aminopyridine, respectively.

Keywords: Local anaesthetics, neuromuscular junction, neuromuscular blocking drugs

Article Details

How to Cite
CARVALHO, Vanessa Henriques et al. Reversal of neuromuscular block produced by local anaesthetics combined with pancuronium. Experimental study.. Medical Research Archives, [S.l.], v. 6, n. 5, may 2018. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/1776>. Date accessed: 19 apr. 2024. doi: https://doi.org/10.18103/mra.v6i5.1776.
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Issue
Vol 6 No 5 (2018): Vol.6 Issue 5 May 2018
Section
Research Articles

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