In vitro cloningand regulatory potential of Bcellhybridomas secreting entero-antigen specific antibody.

Main Article Content

Mogens Claesson Anders Elm Pedersen

Abstract

Enteroantigen specific B cells have previously been demonstrated to play an immunomodulatory role in experimental colitis such as the T cell transfer model of colitis and regulatory B cells are known to be important for suppressing immunopathology in human IBD. Establishing immortalized B cell hybridomas with enteroantigen specificity would be an important tool to investigate further the role of B cells in the immunopathology of experimental colitis and inflammatory bowel disease. Here, based on polyethylene glycol fusion of X63 cells and enteroantigen reactive murine CD19+ B cells, we generated enteroantigen specific B cell hybridomas with proliferative capacity and IL-10 secretion against enteroantigen. The establishment of such hybridomas might be the basis for further assessing the enteroantigen specificity of gut-derived B cells, enteroantigens relevant for experimental colitis as well as human IBD including the immunoregulatory role of such enteroantigen specific B cells.

Article Details

How to Cite
CLAESSON, Mogens; ELM PEDERSEN, Anders. In vitro cloningand regulatory potential of Bcellhybridomas secreting entero-antigen specific antibody.. Medical Research Archives, [S.l.], v. 5, n. Issue 9, sep. 2017. ISSN 2375-1924. Available at: <https://esmed.org/MRA/mra/article/view/1435>. Date accessed: 28 mar. 2024.
Section
Review Articles

References

1. Berthelot, Jean-Marie; Jamin, Christophe; Amrouche, Kahina; Le Goff, Benoit; Maugars, Yves; Youinou, Pierre.2013 "Regulatory B cells play a key role in immune system balance". Joint Bone Spine 80 (1): 18–22. doi:10.1016/j.jbspin.2012.04.010. PMID 22858147. .[17] Next suppressive Breg mechanism is production of transforming growth factor (TGF-β), another anti-inflammatory cytokine.[13]
2. Vitale, G; Mion, F; Pucillo, C.2010 "Regulatory B cells: evidence, developmental origin and population diversity". Molecular immunology 48 (1–3): 1–8. doi:10.1016/j.molimm.2010.09.010. PMID 20950861.
3. Yanaba, K; Bouaziz, JD; Haas, KM; Poe, JC; Fujimoto, M; Tedder, TF.2008 "A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses.". Immunity 28 (5): 639–50. doi:10.1016/j.immuni.2008.03.017. PMID 18482568.
4. Iwata, Y; Matsushita, T; Horikawa, M; Dilillo, DJ; Yanaba, K; Venturi, GM; Szabolcs, PM; Bernstein, SH; Magro, CM; Williams, AD; Hall, RP; St Clair, EW; Tedder, TF.2011 "Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells.". Blood 117 (2): 530–41. doi:10.1182/blood-2010-07-294249. PMID 20962324.
5. van de Veen, W; Stanic, B; Yaman, G; Wawrzyniak, M; Söllner, S; Akdis, DG; Rückert, B; Akdis, CA; Akdis, M.2013 "IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses". The Journal of allergy and clinical immunology 131 (4): 1204–12. doi:10.1016/j.jaci.2013.01.014. PMID 23453135.
6. Wolf, SD; Dittel, BN; Hardardottir, F; Janeway CA, Jr.1996 "Experimental autoimmune encephalomyelitis induction in genetically B cell-deficient mice.". The Journal of experimental medicine 184 (6): 2271–8. doi:10.1084/jem.184.6.2271. PMID 8976182. Janeway´s group observed an immunomodulation of experimental autoimmune encephalomyelitis (EAE) by B cells.[2]
7. Matsushita, Takashi; Yanaba, Koichi; Bouaziz, Jean-David; Fujimoto, Manabu; Tedder, Thomas F. 2008 "Regulatory B cells inhibit EAE initiation in mice while other B cells promote disease progression". Journal of Clinical Investigation. doi:10.1172/JCI36030.
8. Evans, JG; Chavez-Rueda, KA; Eddaoudi, A; Meyer-Bahlburg, A; Rawlings, DJ; Ehrenstein, MR; Mauri, C.2007 "Novel suppressive function of transitional 2 B cells in experimental arthritis.". Journal of immunology (Baltimore, Md. : 1950) 178 (12): 7868–78. PMID 17548625.
9. Mauri, C; Gray, D; Mushtaq, N; Londei, M.2003 "Prevention of arthritis by interleukin 10-producing B cells.". The Journal of experimental medicine 197 (4): 489–501.
10. Tian, J; Zekzer, D; Hanssen, L; Lu, Y; Olcott, A; Kaufman, DL.2001 "Lipopolysaccharide-activated B cells down-regulate Th1 immunity and prevent autoimmune diabetes in nonobese diabetic mice.". Journal of immunology (Baltimore, Md. : 1950) 167 (2): 1081–9. doi:10.4049/jimmunol.167.2.1081. PMID 11441119.
11. Mizoguchi, A; Mizoguchi, E; Smith, RN; Preffer, FI; Bhan, AK.1997 "Suppressive role of B cells in chronic colitis of T cell receptor alpha mutant mice.". The Journal of experimental medicine 186 (10): 1749–56. doi:10.1084/jem.186.10.1749. PMID 9362534.
12. Asseman, C; Mauze, S; Leach, MW; Coffman, RL; Powrie, F.1999. "An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.". The Journal of experimental medicine 190 (7): 995–1004. doi:10.1084/jem.190.7.995. PMID 10510089.
13. El Fassi D, Nielsen CH, Kjeldsen J, Clemmensen O, Hegedüs L. 2008 Ulcerative colitis following B lymphocyte depletion with rituximab in a patient with Graves’ disease. Gut. May;57(5):714–5.
14. Goetz M, Atreya R, Ghalibafian M, Galle PR, Neurath MF. 2007 Exacerbation of ulcerative colitis after rituximab salvage therapy. Inflammatory bowel diseases. Nov;13(11):1365–8.
15. Ronet, C; Hauyon-La Torre, Y; Revaz-Breton, M; Mastelic, B; Tacchini-Cottier, F; Louis, J; Launois, P.2010. "Regulatory B cells shape the development of Th2 immune responses in BALB/c mice infected with Leishmania major through IL-10 production.". Journal of immunology (Baltimore, Md. : 1950) 184 (2): 886–94. doi:10.4049/jimmunol.0901114. PMID 19966209.
16. Douglas, RS; Woo, EY; Capocasale, RJ; Tarshis, AD; Nowell, PC; Moore, JS. 1997. "Altered response to and production of TGF-beta by B cells from autoimmune NZB mice.". Cellular immunology 179 (2): 126–37. doi:10.1006/cimm.1997.1149. PMID 9268496. or systemic lupus erythematosus (SLE).[5]
17. Lundy, SK; Boros, DL.2002. "Fas ligand-expressing B-1a lymphocytes mediate CD4(+)-T-cell apoptosis during schistosomal infection: induction by interleukin 4 (IL-4) and IL-10.". Infection and immunity 70 (2): 812–9. doi:10.1128/iai.70.2.812-819.2002. PMID 11796615.
18. Carter, Laura L.; Leach, Michael W.; Azoitei, Mihai L.; Cui, Junqing; Pelker, Jeffrey W.; Jussif, Jason; Benoit, Steve; Ireland, Gretchen; Luxenberg, Deborah; Askew, G. Roger; Milarski, Kim L.; Groves, Christopher; Brown, Tom; Carito, Brenda A.; Percival, Karen; Carreno, Beatriz M.; Collins, Mary; Marusic, Suzana.2007. "PD-1/PD-L1, but not PD-1/PD-L2, interactions regulate the severity of experimental autoimmune encephalomyelitis". Journal of Neuroimmunology 182 (1–2): 124–134. doi:10.1016/j.jneuroim.2006.10.006. PMID 17182110.
19. Schmidt EG, Larsen HL, Kristensen NN, Poulsen SS, Claesson MH, Pedersen AE. 2012, B cells exposed to enterobacterial components suppress development of experimental colitis. Inflamm Bowel Dis Feb;18(2):284-93. doi: 10.1002/ibd.21769. Epub 2011 May 25. PubMed PMID: 21618359.
20. Venning FA, Trempenau ML, Schmidt E, Claesson MH. 2014 Enteroantigen (eAg)-binding B lymphocytes in the mouse - phenotype, distribution, function and eAg-specificantibody secretion. APMIS. Jul;122(7):616-27.
doi: 10.1111/apm.12200. Epub 2013 Dec 5.,