Main Article Content
Colcemid, a drug initially for arresting cells at mitotic stage, was found to inhibit the rejoining of nucleotide excision repair (NER) in cells exposed to UV irradiation. Subsequent studies reveal not only colcemid but the chemicals which cause oxidative stress have the similar inhibitory effect on gap filling of NER. The inhibitory effect has been correlated to the base excision repair (BER) of oxidative DNA damage and was proposed to result from the competition between BER and NER for common components such as PCNA in the gap filling step. The proposition was supported by the observation that overexpression of PCNA attenuates the oxidative stress-induced inhibition of gap filling of NER. Considering the roles of PCNA in both repairing of oxidative DNA damage and translesion DNA synthesis, a model is proposed. Lastly, the chemistry of colcemid in causing oxidative stress is briefly reviewed.
Authors will be required to fill out the below copyright transfer form during the peer-review process and attach it along with their submission. In return, Knowledge Enterprises Journals grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by Knowledge Enterprises Journals.
of UVC-induced DNA damages in Chinese hamster ovary cells. Mutat Res,
2005. 588(2): p. 118-28.
2. Tzang, B.S., et al., Tuning up or down the UV-induced apoptosis in
Chinese hamster ovary cells with cell cycle inhibitors. Photochem
Photobiol, 2002. 75(6): p. 662-7.
3. Friedberg, E.C., How nucleotide excision repair protects against cancer.
Nat Rev Cancer, 2001. 1(1): p. 22-33.
4. Fujiwara, Y., Postreplication repair of ultraviolet damage to DNA,
DNA-chain elongation, and effects of metabolic inhibitors in mouse L cells.
Biophys J, 1975. 15(5): p. 403-15.
5. Collins, A.R., S.L. Schor, and R.T. Johnson, The inhibition of repair in UV
irradiated human cells. Mutat Res, 1977. 42(3): p. 413-32.
6. Li, P.Y., et al., Antibiotic amoxicillin induces DNA lesions in mammalian
cells possibly via the reactive oxygen species. Mutat Res, 2007. 629(2): p.
7. Arabski, M., et al., Interaction of amoxicillin with DNA in human
lymphocytes and H. pylori-infected and non-infected gastric mucosa cells.
Chem Biol Interact, 2005. 152(1): p. 13-24.
8. Tsai, Y.C., Y.H. Wang, and Y.C. Liu, Overexpression of PCNA Attenuates
Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced
DNA Damage. J Nucleic Acids, 2017. 2017: p. 8154646.
9. Chen, M.K., et al., Delay of gap filling during nucleotide excision repair by
base excision repair: the concept of competition exemplified by the effect of
propolis. Toxicol Sci, 2011. 122(2): p. 339-48.
10. Lu, A.L., et al., Repair of oxidative DNA damage: mechanisms and
functions. Cell Biochem Biophys, 2001. 35(2): p. 141-70.
11. Maynard, S., et al., Base excision repair of oxidative DNA damage and
association with cancer and aging. Carcinogenesis, 2009. 30(1): p. 2-10.
12. Thompson, L.H., et al., A screening method for isolating DNA
repair-deficient mutants of CHO cells. Somatic Cell Genet, 1980. 6(3): p.
13. Dizdaroglu, M., Base-excision repair of oxidative DNA damage by DNA
glycosylases. Mutat Res, 2005. 591(1-2): p. 45-59.
14. Dou, H., et al., Interaction of the human DNA glycosylase NEIL1 with
proliferating cell nuclear antigen. The potential for replication-associated
repair of oxidized bases in mammalian genomes. J Biol Chem, 2008.
283(6): p. 3130-40.
15. Lehmann, A.R., Replication of damaged DNA in mammalian cells: new
solutions to an old problem. Mutat Res, 2002. 509(1-2): p. 23-34.
16. Vaisman, A. and R. Woodgate, Translesion DNA polymerases in
eukaryotes: what makes them tick? Crit Rev Biochem Mol Biol, 2017. 52(3):
17. Hoege, C., et al., RAD6-dependent DNA repair is linked to modification of
PCNA by ubiquitin and SUMO. Nature, 2002. 419(6903): p. 135-41.
18. Risom, L., et al., X-ray-induced oxidative stress: DNA damage and gene
expression of HO-1, ERCC1 and OGG1 in mouse lung. Free Radic Res,
2003. 37(9): p. 957-66.
19. Amorati, R. and L. Valgimigli, Advantages and limitations of common
testing methods for antioxidants. Free Radic Res, 2015. 49(5): p. 633-49.
20. Tsai, Y.C., et al., Induction of oxidative DNA damage by flavonoids of
propolis: its mechanism and implication about antioxidant capacity. Chem
Res Toxicol, 2012. 25(1): p. 191-6.