THG imaging of lipid body profiles in diagnosis of biological samples
Main Article Content
Abstract
Abstract
Non linear optical imaging techniques are at the forefront of biomedical research over the last years. The ability of label free imaging, diffraction limited resolution, high penetration depth, intrinsic three dimensional (3D) sectioning and low phototoxicity make this technology a perfect diagnostic tool in many biomedical applications. This review briefly refers to the non linear microscopy modalities, depicts their advantages and focuses on recent applications of third harmonic generation microscopy in recognizing lipid body structures in biological specimens. Specifically, this study concentrates on the efficiency of THG signal quantification to be used as an innovative diagnostic tool in the follow up of mouse embryo development, in distinguishing mouse BV2 microglia cell activation and allowing classification of human breast cancer cells.
Article Details
How to Cite
GAVGIOTAKI, Evangelia et al.
THG imaging of lipid body profiles in diagnosis of biological samples.
Medical Research Archives, [S.l.], v. 4, n. 7, nov. 2016.
ISSN 2375-1924.
Available at: <https://esmed.org/MRA/mra/article/view/619>. Date accessed: 18 apr. 2024.
Keywords
Third harmonic Generation, Label free imaging, Lipid Bodies , Non linear Microscopy Quantification analysis, Mouse embryos , BV2 glial cells , Breast cancer cells
Section
Review Articles
The Medical Research Archives grants authors the right to publish and reproduce the unrevised contribution in whole or in part at any time and in any form for any scholarly non-commercial purpose with the condition that all publications of the contribution include a full citation to the journal as published by the Medical Research Archives.
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Chen, S.-Y., Wu, H.-Y., & Sun, C.-K. (2009). In vivo harmonic generation biopsy of human skin. Journal of Biomedical Optics, 14(6), 060505-060505-060503. doi: 10.1117/1.3269676
Chiotaki, R., Polioudaki, H., & Theodoropoulos, P. A. (2014). Differential nuclear shape dynamics of invasive andnon-invasive breast cancer cells are associated with actin cytoskeleton organization and stability. Biochem Cell Biol, 92(4), 287-295. doi: 10.1139/bcb-2013-0120
Chu, S. W., Chen, S. Y., Tsai, T. H., Liu, T. M., Lin, C. Y., Tsai, H. J., & Sun, C. K. (2003). In vivo developmental biology study using noninvasive multi-harmonic generation microscopy. Opt Express, 11(23), 3093-3099.
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Daemen, S., van Zandvoort, M. A. M. J., Parekh, S. H., & Hesselink, M. K. C. (2016). Microscopy tools for the investigation of intracellular lipid storage and dynamics. Molecular Metabolism, 5(3), 153-163. doi: http://dx.doi.org/10.1016/j.molmet.2015.12.005
Débarre, D., & Beaurepaire, E. (2007). Quantitative Characterization of Biological Liquids for Third-Harmonic Generation Microscopy. Biophysical Journal, 92(2), 603-612. doi: 10.1529/biophysj.106.094946
Débarre, D., Olivier, N., Supatto, W., & Beaurepaire, E. (2014). Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range. PLoS ONE, 9(8), e104250. doi: 10.1371/journal.pone.0104250
Debarre, D., Supatto, W., Farge, E., Moulia, B., Schanne-Klein, M. C., & Beaurepaire, E. (2004). Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos. Opt Lett, 29(24), 2881-2883.
Debarre, D., Supatto, W., Pena, A. M., Fabre, A., Tordjmann, T., Combettes, L., Beaurepaire, E. (2006). Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy. Nat Methods, 3(1), 47-53. doi: 10.1038/nmeth813
Digel, M., Ehehalt, R., & Füllekrug, J. (2010). Lipid droplets lighting up: Insights from live microscopy. FEBS Letters, 584(11), 2168-2175. doi: http://dx.doi.org/10.1016/j.febslet.2010.03.035
Gavgiotaki, Filippidis, Kalognomou, Tsouko, Skordos, Fotakis, & Athanassakis. (2015). Third Harmonic Generation microscopy as a reliable diagnostic tool for evaluating lipid body modification during cell activation: The example of BV-2 microglia cells. Journal of Structural Biology, 189(2), 105-113. doi: http://dx.doi.org/10.1016/j.jsb.2014.11.011
Gavgiotaki, Filippidis, G., Psilodimitrakopoulos, S., Markomanolaki, H., Kalognomou, M., Agelaki, S., .Athanassakis, I. (2015). Third Harmonic Generation microscopy as a diagnostic tool for the investigation of microglia BV-2 and breast cancer cells activation. Proc. SPIE 9536, Advanced Microscopy Technique IV, and Neurophotonics II, 953614 (July 14, 2015), doi:10.1117/12.2183286; http://dx.doi.org/10.1117/12.2183286
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