Comparative phytochemical and antimicrobial activities of polar solvents tuber extracts of Icacina senegalensis A. Juss (Icacinaceae)

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Esien David-Oku Stella Celestine Bassey Juliet Ifeoma Obiajunwa-Otteh Emem Udoma Ekpenyong


Aim: This study was designed to assess phytochemical and antimicrobial activities of methanol (Met), aqueous (Aq), and methanol-aqueous (Met:Aq) extracts of Icacina senegalensis tuber.

Methodology: Phytochemical screening of the various tuber extracts was performed using standard methods. Antimicrobial testing was performed on clinical isolates of Staphylococcus aureus, Shigella dysenteriae, Salmonella typhi, Peudomonas aeroginosa, Serratia marcescens, and Escherichia coli using the well diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the extracts were determined by the tube dilution method.

Results: The following phytochemicals were detected: alkaloids, phenols (all extracts), phytate, oxalate (Met), saponin (Aq), steroids, and cardiac glycosides (Met:Aq). All three extracts displayed good antibacterial activities against the test organisms.

Aq had the least MIC (1.41-11.25 μg/mL) and MBC (5.63-22.5 μg/mL) against the test organisms. Similar values for Met and Met:Aq were (3.125-6.25; 12.5-50 μg/mL) and (3.125-25; 25-50 μg/mL) respectively. Therefore the antibacterial potencies of the extracts seemed to be in the order of Aq ˃ Met˃ Met:Aq.

Conclusion: The bioactive (phytochemical) components of the different polar solvents extracts of Icacina senegalensis tuber possess wide spectrum antibacterial potency. Thus, a combination of these extracts in antibacterial drug formulation is an attractive option for enhanced efficacy.

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How to Cite
DAVID-OKU, Esien et al. Comparative phytochemical and antimicrobial activities of polar solvents tuber extracts of Icacina senegalensis A. Juss (Icacinaceae). Medical Research Archives, [S.l.], v. 5, n. 11, nov. 2017. ISSN 2375-1924. Available at: <>. Date accessed: 15 dec. 2017. doi:
Icacina senegalensis, tuber, phytochemical composition, antibacterial agent, methanol, aqueous
Research Articles


[1]. Kunle FO, Egharevba OH, Ahmadu OP. Standardization of herbal medicines - A review. 2012;4:101–12.
[2]. Batista R, de Jesus Silva Júnior A, Braga de Oliveira A. Plant-Derived Antimalarial Agents: New Leads and Efficient Phytomedicines. Part II. Non-Alkaloidal Natural Products. Molecules. 2009;14:3037–72.
[3]. Oliveira AB, Dolabela MF, Braga FC, Jácome RLRP, Varotti FP, Póvoa MM. Plant-derived antimalarial agents: New leads and efficient phythomedicines. Part I. alkaloids. An Acad Bras Cienc. 2009;81(4):715–40.
[4]. Cao L, Si JY, Liu Y, Sun H, Jin W, Li Z, et al. Essential oil composition , antimicrobial and antioxidant properties of Mosla chinensis Maxim. Food Chem. 2009;115(3):801–5. Available from:
[5]. Pandey S, Seth A, Tiwari R, Singh S, Behl HM, Singh S, et al. Development and evaluation of antimicrobial herbal cosmetic preparation. African J Pharm Pharmacol. 2014;8(20):514–28.
[6]. European Medicines Agency. Guideline on specifications : test procedures and acceptance criteria for herbal substances, herbal preparations and herbal medicinal products / traditional herbal medicinal products. London; 2011.
[7]. Obiajunwa-Otteh J, Akuodor GC, Onyekpe PI, Umoh EU. Phytochemical and Antimicrobial potency of Aqueous and Methanol Leaf Extracts of Icacina senegalensis. Pharmacologia. 2014;5(8):321–5.
[8]. Sarr SO, Perrotey S, Fall I, Ennahar S, Zhao M, Diop YM, et al. Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity. Malar J. 2011;10:85.
[9]. Fay JM. Icacina-Oliviformis (Icacinaceae) - a Close Look at an Underexploited Food Plant .3. Ecology and Production. Econ Bot. 1993;47:163–70. Available from: ://A1993LJ71900005
[10]. David-Oku E, Obiajunwa-Otteh JI, Akuodor GC. Evaluation of the antimalarial potential of I cacina senegalensis J uss. Asian Pac J Trop Med. 2014;4(Suppl 2):S819–22.
[11]. Akuodor GC, Udia PM, Bassey A, Chilaka KC, Okezie OA. Antihyperglycemic and antihyperlipidemic properties of aqueous root extract of Icacina senegalensis in alloxan induced diabetic rats. J Acute Dis. 2014;2014:99–103.
[12]. David-Oku E, Obiajunwa-Otteh JI. A combination of the tuber and leaves of Icacina senegalensis A. juss (Icacinaceae) improves the antimalarial activity of the plant in mice. J Coast Life Med. 2015;In press.
[13]. Ajayi IA, Ajibade O, Oderinde RA. Preliminary phytochemical analysis of some plant seeds. Res J Chem Sci. 2011;1(3):58–61.
[14]. Akuodor GC, Essien AD, Ibrahim JA, Bassey A, Akpan JL, Okoro NC, et al. Phytochemical and antimicrobial properties of the methanolic extracts of Bombax buonopozense leaf and root. Asian J Med Sci. 2011;2(2):190–4.
[15]. Adegoke AA, Adebayo-Tayo BC. Antibacterial activity and phytochemical analysis of leaf extracts of Lasienthera africanum. African J Biotecnol. 2009;8(1):077–80.
[16]. Mbatchou VC, Dawda S. Phytochemical and pharmacological profile of genus Icacina. Phytopharmacology. 2012;2(2):135–43.
[17]. Dei HK, Bacho A, Adeti J, Rose SP. Nutritive value of false yam (Icacina oliviformis) tuber meal for broiler chickens. Poult Sci. 2011;90:1239–44.
[18]. Edeoga HO, Okwu OE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African J Biotechnol. 2005;4(7):685–8.
[19]. Timbrell JA. Principles of Biochemical Toxicology. 4th ed. New York: Informa Healthcare USA, Inc; 2009. 239 p.
[20]. Ginsburg H, Deharo E. A call for using natural compounds in the development of new antimalarial treatments – an introduction. Malar J. 2011;10(Suppl 1):S1. Available from:
[21]. Akuodor GC, Essien AD, David-Oku E, Chilaka KC, Akpan JL, Ezeokpo B, et al. Gastroprotective effect of the aqueous leaf extract of Guiera senegalensis in Albino rats. Asian Pac J Trop Med. 2013;6(10):771–5.
[22]. Maimoona A, Naeem I, Saddiqe Z, Jameel K. A review on biological, nutraceutical and clinical aspects of French maritime pine bark extract. J Ethnopharmacol. 2011;133(2):261–77. Available from:
[23]. Qassim A. Antibacterial potential of some plants of traditional use in India against pathogenic strains of S. aureus. J Coast Life Med [Internet]. 2015;3(3):204–10. Available from:
[24]. Smit N, Vicanova J, Pavel S. The hunt for natural skin whitening agents. Int J Mol Sci [Internet]. 2009 Dec [cited 2014 Aug 16];10(12):5326–49. Available from: ype=abstract
[25]. Onguéné A, Onguéné PA, Ntie-kang F, Lifongo LL, Ndom JC, Sippl W. The potential of anti-malarial compounds derived from African medicinal plants . Part I : A pharmacological evaluation of alkaloids and terpenoids. Malar J. 2013;12:449.

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